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«Notes on Spanish glassy-haired Hyaloscyphaceae A. Raitviir1 & R. Galän Department of Plant Biology, University of Alcalä de Henares, Alcalä de ...»

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Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.a

Notes on Spanish glassy-haired Hyaloscyphaceae

A. Raitviir1 & R. Galän

Department of Plant Biology, University of Alcalä de Henares, Alcalä de Henares

(Madrid), Spain

Raitviir, A. & R. Galän. (1993). Notes on Spanish glassy-haired Hyaloscypha-

ceae. - Sydowia 45(1): 34-54.

Nine species of the Hyaloscyphaceae belonging to three genera with glassy

hairs are described from Spain. Four of them, Mollisina minutissima, Urceolella ma- gnified, Urceolella pinicola and Urceolella triseptata are new. Hyalopeziza verna and Urceolella graddonii are proposed as a new combination and as a new name, respectively. All species are described and illustrated and their taxonomic position is discussed.

Keywords: Ascomycetes, Discomycetes, Hyaloscyphaceae, taxonomy.

Because of their attractive morphology and histochemistry, the glassy-haired Hyaloscyphaceae have received special attention by a number of scientists following the work of Raschle (1977). Korf & Kohn (1980) stressed the importance of histochemical reactions in the investigation of hair morphology and this line was followed by Huhti- nen (1987b). We agree that the separation of Urceolella Boud. from Hyalopeziza Fuckel on the basis of hair and ectal excipulum reactions in KOH and Congo Red has advantages over the taxonomy used by Raitviir (1970). Also, it is evident that the dextrinoid or amyloid reac- tions in Melzer's reagent can be used only at the species level, and for this reason we treat Unguicularia Höhn, as a synonym of Hyalopeziza Fuckel.

The present paper deals with the species collected by the authors during the senior author's sabbathical at the University of Alcalä de Henares. The genus Protounguicularia Raitv. & Galan described re- cently by the authors (Raitviir & Galän, 1986), and discussed in detail also by Huhtinen (1987a), is not treated here.

Material and methods Several glassy-haired species as well as some other hyaloscypha- ceous fungi have very minute apothecia which are difficult to notice 1 Present address: Institute of Zoology and Botany, 21 Vanemuise Street, EE-2400 Tartu, Estonia 34 Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.a in the field. The actual number of species of the Hyaloscyphaceae present at any collecting site can be more accurately determined by scanning a large amount of substrata under a dissecting microscope. The use of such a technique has been explicitly stated only for the discovery of Trichodiscus diversipilus by Graddon (1977) but was applied more widely by this author. In our studies 3 of 4 new species, namely Mollisina minutissima, Urceolella pinicola and Urceolella triseptata were discovered by scanning freshly collected forest debris in the laboratory under a dissecting microscope.

In this study 5% potassium hydroxide (KOH) was used to rehydrate dry apothecia. Melzer's reagent (MLZ), Cotton Blue (CB) and Congo Red (CR) were used in the same way as described by Huhtinen (1987b). Photographs were taken with a Nikon Labophot-2 phasecontrast microscope. Line drawings were made using a Carl Zeiss Jenaval phase-contrast microscope equipped with a drawing tube.

Description of species

1. Hyalopeziza millepunctata (Lib.) Raitv., Scripta Mycologica 1: 34.

1970.-Figs. 1: A, 2: E-H.

Bas.: Peziza millepunctata Lib. PI. Crypt. Ard. Fasc. 2, No. 128. 1832.

= Unguicularia millepunctata (Lib.) Dennis, Mycol. Papers 32: 79. 1949.

= Unguicularia scrupulosa (Karst.) Höhn., Sitzungsber. Akad. Wiss. Wien, Math.-Nat. Cl. 115: 1279. 1906.

= Peziza scrupulosa Karst., Not. Sällsk. Fauna Flora Fenn. Förh. 10: 178. 1869.

For full synonymy see Raschle (1977).

A p o t h e c i a scattered to gregarious, sessile. Disc 0.2-0.3 mm in diameter, whitish. - R e c e p t a c l e urceolate, whitish to pale greyish when fresh, at the margin minutely downy, whitish gray or pale olivaceous gray to brownish-gray with whitish downy margin when dry.

Ectal excipulum consisting of hyaline, prismatic cells 8-12 x 3-5 urn, having thin to firm walls, remaining hyaline in MLZ and CR; the space between the cells is filled with intercellular resinous matter which encrusts the excipular cell walls and is responsible for the colouring of excipulum and stains yellowish brown to golden brown in KOH. H a i r s cylindrical to cylindric-conical with broadly rounded tip, hyaline, 10-35 x 3-5 jam with thin walls in basal part and solid glassy apex not losing refractiveness in KOH, staining deep reddish-brown in MLZ, and deep red in CR. - A s c i clavate, 30-36 x 4-5 urn, arising from simple septa, apical pore blue in MLZ, with biseriate, irregularly biseriate or obliquely uniseriate arrangement of spores. - A s c o s p o r e s ellipsoid, slightly inequilateral, sometimes clavate-ellipsoid, hyaline, aseptate, usually containing two minute polar guttules, 6-7.5 x 1.6-2.2 urn. - P a r a p h y s e s filiform, rarely branching in basal part, obtuse, not exceeding the asci, 1 urn in diameter.

35 Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.a

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.11 Fig. 1. - A. Hyalopeziza millepunctata (RG 6681): Excipulum with hairs. - B-C.

Hyalopeziza verna (TAA-136847): B. Excipulum with hairs. - C. Portion of hymenium showing asci with spores. - D-H. Hyalopeziza pygmaea (TAA-136844): D-E.





Granulated hairs. - F. Spores. - G. Part of excipulum. - H. Part of excipulum and hairs stained in CR.

Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.a

–  –  –

38 Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.a Fig. 2. - A-D. Hyalopeziza pygrnaea (TAA-136844): A. Excipular cell with marginal

hairs. - B. Spores. - C. Asci. - D. Differently shaped hairs, two of them showing granulation. - E-H. Hyalopeziza millepunctata (RG 6681): E. Ascus and paraphysis. F. Excipular cells. - G. Hair. - H. Spores. - I-L. Hyalopeziza verna (TAA-136847):

I. Asci. - J. Hair showing granulated thin wall at its base. - K. Margin of ectal excipulum with hairs. - L. Spores.

–  –  –

40 Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.a R. Galan & A. Raitviir (RG-6658, TAA-136845); on fallen leaves of Populus sp., between Naharros and Robledo de Corpes, Guadalajara, 18. 11. 1991, M. de la Cruz (RG-6713).

The species was fruiting abundantly on fallen leaves of poplar and willow in Anquela del Ducado. A part of the material was sent to

–  –  –

Fig. 3 - Mallisinu flava (RG 6t58). A B Hairs with characteristic finger-shaped branches. - C. Asci with spores and part of ectal excipulum.

Dr. Arendholz, who kindly confirmed our identification (Arendholz, pers. comm.).

Arendholz (1979) has described the ascus pore of Mollisina flava as inamyloid but after KOH pretreatment the ascus pore stains deep blue in MLZ.

This is the first record of the species from Spain.

5. Mollisina minutissima Raitv. & Galan sp. nov. - Figs. 4: A-C, 5:

. A-D.

Apothecia gregaria, subsessilia vel substipitata, 0.2 mm diametro, cupulata vel patelliformia, margine puberula, 0.2 mm diametro, albida vel pallide cremea, sicca ochracea. Excipulum ectale e cellulis prismaticis hyalinis compositum. Pili cylindracei vel subclavati, apicibus ramosis, 20-40 x 3-5 urn. Asci cylindraceo-clavati vel clavati, octospori, in basi non uncinati, poro iodo non eoerulescenti, 38-50 x 4.5-6.5 urn. Sporae ellipsoideae, hyalinae, aseptatae, 6-7.5 x 2-2.7 um. Paraphyses filiformes, ascos non superantes, 1.2-1.5 um, apicibus 2 um in diametro.

Ad ramos siccos Cisti ladaniferi L.

–  –  –

branches up to 3.5 um long, solid when intact but losing the solidness in. KOH. - A s c i cylindric-clavate to clavate, 38-50 x 4.5-6.5 urn, arising from simple septa, apical pore not blue in MLZ, with irregularly biseriate to obliquely uniseriate arrangement of spores. - A s c o s p o r e s ellipsoid, usually slightly inequilateral, hyaline, aseptate, eguttulate, 6-7.5 x 2-2.7 um. - P a r a p h y s e s filiform, sparsely branching, obtuse to very slightly swollen, not exceeding the asci, 1.2—1.5 um, apically up to 2 urn in diameter.

–  –  –

On decaying small branches of Cistus ladanifer L.

S p e c i m e n s e x a m i n e d. - On a fallen thin branch of Cistus ladanifer L., junction of roads Tamajön-Puebla de Valles, Guadalajara, 3. 12. 1991, A. Raitviir & R. Galan (Holotype in TAA-136846); on cupule protecting scales of Cistus ladanifer L., Carrascosa de Henares, Guadalajara, 3. 1. 1992, M. de la Cruz (RG-6729).

This species differs from all described species of Mollisina Höhn, and Dendrotrichoscypha Svr. The foliicolous species Mollisina uncinata Arendholz & R. Sharma has longer asci and comparatively wide ellipsoid spores.

6. Urceolella magnifica Raitv. & Galan sp. nov. - Figs. 6: A-H, 7: A-E.

Apothecia substipitata, albida, hymenio pallide ochraceo griseo, 0.3-0.8 mm diametro, cupulata vel patelliformia, extus longe niveopilosa. Pili cylindracei, apici

–  –  –

Fig. 6. - Urceolella magnified (TAA-136848): A-C. 4-spored asci. - D. 8-spored ascus. - E. Branching paraphysis. - F-G. Hairs. - H. Part of ectal excipulum.

–  –  –

Fig. 8. - Urceolella pinicola (TAA-i:i(5H49).: A. Marginal hairs. - B. Ectal excipulum.

- C. Hair. - D. Asci with spores. - E. Branching paraphysis. - F-H. Asci showing the characteristic basal hook. - I. Asci with apical pore blue in MLZ.

Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.a

–  –  –

8. Urceolella graddonii Raitv. & Galan nom. nov. - Figs. 10: A-B.

= Hyalotricha salicicola Graddon, Trans. Br. mycol. Soc. 69: 262. 1977.

= Urceolella salicicola Raschle, Sydowia 39: 215. 1977.

A p o t h e c i a in small groups on the lower surface of the leaf blade, substipitate. Disc 0.2-0.3 mm in diameter, white. - R e c e p t a c l e deeply cupulate to almost infundibuliform, urceolate and almost closed with enrolled margins when dry, pure snow-white, densely covered, by pure white, long, undulating hairs. Ectal excipulum well developed, consisting of a few layers of hyaline, thin-walled, prismatic cells 9-20 x 6-8 |_im, staining deep red in CR. - H a i r s cylindrical to slightly tapering toward the apex, flexuous, hyaline, smooth, 80-140 x 2.5-3.5 um, with thick to very thick, more or less evenly thickened walls losing their refractiveness in KOH, remaining hyaline in CR and MLZ, aseptate. - A s c i cylindric-clavate, 27-34 x 4-4.5 um arising from croziers, apical pore blue in MLZ, 8-spored, with irregularly uni- to biseriate arrangement of spores. - A s c o

–  –  –

ceolella salicicola (Graddon) Raitv. & Galan would be an illegitimate name because of the earlier homonym Urceolella salicicola Raschle.

Therefore we propose a new name for this species.

U. graddonii, although not common, seems to be widely distributed and to grow on a wide range of hosts. The first author has collected it in Estonia. Baral & Krieglsteiner (1985) and Beyer (1991) have reported it from Germany on Quercus, Salix and Betula leaves.

9. Urceolella triseptata Raitv. & Galan sp. nov. - Figs. 11: A-E, 12:

A-E.

Apothecia minutissima, subsessilia, albida, margine breviter pilosa. Pili subconici, supracrassiter tunicati, 1-4-septati, 20-40 x 3-5 |im. Excipulum ectale e cellulis prismaticis hyalinis tenuiter tunicatis compositum. Asci cylindraceo-clavati, octospori, poro iodo coerulescenti praediti, 40-50 x 5-9 urn. Ascosporae cylindraceoclavatae vel clavatae, hyalinae, triseptatae, 14-17 x 2.5-3.5 |im. Paraphyses filiformes, ascos non superantes, 1 um diametro. Species ab aliis generis sporis magnis triseptatis differt. In cupulis dejectis Quercus crescit.

Holotypus: in cupula dejecta Quercus rotundifoliae Lam., Ermita de los Enebrales, Tamajön, Guadalajara, Hispania, 03. 12. 1991, A. Raitviir & R. Galan (TAA

<

E t y m o l o g y. - Referring to the 3-septate spores.

A p o t h e c i a gregarious, subsessile to substipitate, very small.

Disc 0.2 mm in diameter, whitish to pale cream-coloured.

- R e c e p t a c l e shallow cupulate to saucer-shaped, concolorous with the hymenium, marginally covered by short, stiff, white hairs. Ectal excipulum very thin, consisting of only one layer of hyaline, thin-walled, prismatic cells 8-12 x 3-4.5 um, staining deep red in CR, remaining hyaline in MLZ. - H a i r s conical to cylindric-conical, hyaline, 20-40 x 3-5 urn, with walls up to 2 |im thick, walls losing refractiveness in KOH, not staining in MLZ and CR, 1-4-septate with very thick septa.

- A s c i cylindric-clavate, 40-50 x 5-9 fim, arising from simple septa, apical pore blue in MLZ, with irregularly biseriate to obliquely uniseriate arrangement of spores. - A s c o s p o r e s cylindric-clavate to clavate, hyaline, distinctly 3-septate, 14-17 x 2.5-3.5 urn. - P a r a p h y s e s slender filiform, branching, obtuse, not exceeding the asci, about 1 urn in diameter.

S p e c i m e n e x a m i n e d. - On a fallen cupule of Quercus rotundifolia Lam., Ermita de los Enebrales, Tamajön, Guadalajara, Spain, 03. 12. 1991, A. Raitviir & R. Galan (Holotype in TAA-136850).

–  –  –

Acknowledgments The authors are grateful to Mr. Marcelino de la Cruz for providing specimens, Mr. Luis Monje for photographic assistance and Mrs. Malle Raitviir for inking the line drawings. The junior author wishes to express his gratitude to the Vicerrectorado de Investigacion of the University of Alcalä de Henares for the concession of the project no. 90/B7 which made possible the acquisition of new microscopical instruments.

References

Arendholz, W.-R. (1979). Morphologisch-taxonomische Untersuchungen an blattbewohnenden Ascomyceten aus der Ordnung Helotiales. - Ph. D. Thesis, Univ.

Hamburg. 115 pp.



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