«Curriculum Vitae, July 26, 2010, page 1 CURRICULUM VITAE I. NAME AND ADDRESS Name: David Michael Gilbert, Ph.D. Current office address: 319 Stadium ...»
Gilbert, D.M., Curriculum Vitae, July 26, 2010, page 1
I. NAME AND ADDRESS
Name: David Michael Gilbert, Ph.D.
Current office address: 319 Stadium Dr. King Building, Room 3066
Department of Biological Science
Florida State University
Tallahassee, FL 32306-4295
Tel.: (850) 645-7583 FAX: (850) 645-8447
II. EDUCATION AND DEGREESB.A., 1982 University of California at San Diego: Revelle College Major: Biochemistry/Cell Biology; Minor: Philosophy Undergraduate Honors Thesis Advisor: Dr. Immo Scheffler Thesis title: "Enzyme Induction in a Temperature Sensitive Chinese Hamster Cell Mutant Deficient in RNA Metabolism."
Ph.D., 1989 Stanford University Department of Genetics Thesis advisor: Dr. Stanley N. Cohen Thesis title: "Temporal Order of DNA Replication in Mammalian Cells: Its Relationship to Gene Expression."
III. FELLOWSHIPS / POST-DOCTORAL TRAINING1990 - 1991 Post-doctoral training, C.N.R.S. (EMBO long-term fellowship) Faculté de Médecine, Strasbourg, FRANCE Subject of research: Mechanism of transcriptional induction by the human estrogen receptor expressed in yeast.
Laboratory: Dr. Pierre Chambon 1991 - 1994 Roche Institute of Molecular Biology (Roche Fellowship) Subject of Research: Initiation of DNA Replication in Mammalian Cells.
Laboratory: Dr. Melvin DePamphilis
IV. ACADEMIC AND RESEARCH POSITIONS1994 - 1998 Assistant Professor Department of Biochemistry and Molecular Biology State University of New York Health Sciences Center (SUNY HSC) 1998-2003 Associate Professor Department of Biochemistry and Molecular Biology State University of New York Upstate Medical University (formerly SUNY HSC) 2003-present Professor Department of Biochemistry and Molecular Biology State University of New York Upstate Medical University 2006-present J. Herbert Taylor Distinguished Professor of Molecular Biology Department of Biological Science Florida State University Gilbert, D.M., Curriculum Vitae, July 26, 2010, page 2
V. PROFESSIONAL HONORS2010 Elected Council Delegate of the AAAS, section on Biological Sciences 2008 Elected Fellow of the AAAS 2005 Selected to compete in Phase II of the NIH Director's Pioneer Award (NDPA) 2004 Nominated as a Howard Hughes Medical Institute Investigator 2004 NIH Career Enhancement (K18) Award for Stem Cell Research SUNY Upstate President’s Award for Excellence in Research by a Young Investigator 1991 - 1994 Roche Institute of Molecular Biology Post-Doctoral Fellow 1989 - 1991 European Molecular Biology Organization Post-Doctoral Fellow 1990 NATO Post-Doctoral Fellowship Award, declined 1982 Honors with Distinction, University of California
October, 1994 Montreal Eukaryotic DNA Replication Meeting (selected) Sept. 1995 Cold Spring Harbor: "Eukaryotic DNA Replication" (selected) May 1997 Buffalo DNA Replication Symposium (selected) June 1997 CNRS Jacques Monod Conference: "Regulation of DNA Replication in Prokaryotes and Eukaryotes: Molecular Aspects", Aussois, France (selected) April, 1998 Keystone: "The Nuclear Matrix" (invited) May, 1998 Buffalo DNA Replication Symposium (selected) July, 1998 Gordon Conference: "Biological Regulatory Mechanisms" (invited) August, 1998 Chair: Session on Initiation of DNA Replication at the XVIIIth International Congress of Genetics, Beijing, China (invited) May, 1999 HUGO Workshop on Genome Organization, Edinburgh (invited) May, 2000 Michigan State University Genetics Symposium (invited) Sept., 2000 Salk Institute: Eukaryotic DNA Replication (selected) Dec., 2000 Japanese Molecular Biology Society (invited) June, 2001 Buffalo DNA Replication Symposium (selected) Oct. 2001 AACR: "Cancer and Chromosomal Organization" (invited) Feb. 2002 23rd Lorne Genome Conference, Australia (invited) June, 2002 Buffalo DNA Replication Symposium (selected) August, 2002 Salk Institute: Eukaryotic DNA Replication (selected) Sept., 2002 Cold Spring Harbor: "Dynamic Structure of the Nucleus" (selected) Oct. 2002 5th International Symposium of Molecular Medicine, Crete, Greece (invited) FASEB: 52nd Ann. Mtg. of the Am. Soc. of Hum. Gen. (invited) Oct. 2002 Jan. 2003 Keystone Chromatin Meeting (invited) April, 2004 Marie Curie Research Institute Workshop on Chromatin and DNA Replication (invited) April, 2004 Eastern Great Lakes Molecular Evolution VIII (selected) Oct. 2004 EMBO workshop on Nuclear organization; Elmau (invited) Dec., 2004 Japanese Molecular Biology Society (invited) June, 2005 Buffalo DNA Replication Symposium (selected) June, 2005 FASEB Meeting on Nuclear Structure and Cancer (invited) Session Chair, Cold Spring Harbor: “Eukaryotic Chromosome Replication” (invited) Sept. 2005 Sept., 2005 Session Co-chair, EMBO Conf.: Nuclear Structure and Dynamics, Montpellier (invited) Oct. 2005 Cell-cycle Symposium, Tokyo (invited) Aug., 2006 Session Chair, Salk Institute: DNA Replication and Genome Integrity (invited) Session Chair, Cold Spring Harbor: “Dynamic Structure of the Nucleus” (invited) Sept., 2006 Gilbert, D.M., Curriculum Vitae, July 26, 2010, page 3 Feb. 2007 Workshop on Epigenetics and Evolution (co-organizer) April, 2007 eIMBL workshop on DNA replication, Tokyo (invited) June, 2007 FASEB Meeting on Nuclear Structure and Cancer June 2007 Buffalo DNA Replication Symposium (selected) Cold Spring Harbor: “Eukaryotic Chromosome Replication” (selected) Sept. 2007 July, 2008 Shanghai Symposium on Epigenetics in Development and Diseases (invited) HHMI Janelia Farms “Chromatin Mechanisms in Pluripotency” (selected) Oct. 2008 Oct. 2008 Southeastern Stem Cell Consortium First Annual (Invited) NCI Symposium, “Genome-wide Chromatin Structure and Function” (invited) Oct., 2008 Feb., 2009 Int. Symp. on Epigenetic Modifications of the Genome, Hyderabad, India (invited) Feb., 2009 Keystone: Chromatin Dynamics and Higher Order Organization (invited) April, 2009 ASBMB Experimental Biology (selected) June 2009 FASEB Meeting on Nuclear Structure and Cancer (invited) Sept. 2009 KEYNOTE SPEAKER: New Developments in Stem Cell Science (Melbourne) 3rd NIGMS Workshop on Human Embryonic Stem Cell Research (invited) Sept. 2009 Dec. 2009 Japanese Molecular Biology Society, Yokohama (invited) Feb. 2010 35 yrs of DNA cloning: Individual and Global Health Priorities in the 21st Century (invited) June 2010 Cold Spring Harbor Symposium on Quantitative Biology: The Cell Nucleus (invited) July 2010 Gordon Conference on Chromatin Structure and Function (invited) Sept. 2010 Wellcome Trust Signaling to Chromatin Conference (invited)
Too many to list; 3-6 papers reviewed per month for many journals such as Cell, Science, Nature, PNAS.
Grant Peer Review Committees:
’95-present National Science Foundation (ad hoc) 1995-98 US Army Breast Cancer Research Program (Molecular Genetics) 1997 NIH (Molecular Cytology Study Section) 1998-04 American Cancer Society (Cell-Cycle and Growth Control) 99-00 NIH RO1 (Biochemistry) and PPG ’98-present Wellcome Trust ’99-present HFSP International Projects ’00-present Cancer Research Campaign: 5yr. reviews / Infrastructure grants / Project grants ’01-present The Israel Science Foundation ’01-present Istituto Pasteur-Fondazione Cenci Bolognetti 03 NIH (CDF-2) 07 NIH (MGB) 08 NIH ENCODE Special Emphasis Panel 08 NIH Career Enhancement Panel (K18) 09 Challenge Grant review (x 5)
COMPLETED "Assembly of the Replication Initiation Complex" Principle Investigator: David M. Gilbert, PhD Agency: Milheim Foundation Type: Research Grant (95-28) Period: 7/1/95 – 6/30/96 Total Costs: $10,000 The overall objective of this project was to determine what governs the assembly of DNA replication initiation complexes in mammalian cells.
"Nuclear Structure and Metazoan Origins of Replication" Principle Investigator: David M. Gilbert, PhD Agency: National Science Foundation Type: Research Project Grant (MCB-9505907) Period: 9/15/95 - 8/31/98 Total Costs: $390,000 This project examined the requirements for site-specific initiation of replication in Xenopus egg extracts.
"Developmental Regulation of DNA Replication" Principle Investigator: David M. Gilbert, PhD Agency: March of Dimes Type: Research Grant Period: 7/01/96 - 5/31/00 Total Costs: $204,000 The goal of this project was to determine whether the temporal order of DNA replication is maintained when mammalian cell nuclei are introduced into Xenopus egg extract.
"Nuclear Structure and Metazoan Origins of Replication" Principle Investigator: David M. Gilbert, PhD Agency: NIH Type: RO1 (R01GM57233-01) Period: 9/01/98 - 8/31/02 Total Costs: $881,044 The overall objective of this grant was to achieve a description of replication-relevant changes in nuclear architecture that take place as the nucleus matures during early G1-phase. This grant also developed various cell biological tools to examine the assembly of replication factories.
"The Mammalian Origin Recognition Complex" Principle Investigator: David M. Gilbert, PhD Agency: American Cancer Society Type: Research Project Grant (CCG-85791) Period: 7/01/00 - 6/30/04 Total Costs: $915,000.00 This project studied the assembly and activities of the origin recognition complex proteins.
This project examined how the replication timing program is established in mammalian cells.
“Developmental Regulation of DNA Replication” Principle Investigator: David M. Gilbert, PhD Agency: Hendrick’s Foundation Type: Institutional Pilot Project Grant Period: 1/1/03-12/31/03 Total Costs: $15,000 This was a pilot grant to purchase equipment and supplies necessary to study the developmental control of DNA replication during the differentiation of pluripotent mouse embryonic stem (ES) cells.
“DNA Replication During Differentiation of ES Cells” Principle Investigator: David M. Gilbert, PhD Agency: NIH Type: K18 Period: 07/01/04-08/31/05 Total Costs: $250,324 This was a Career Enhancement Award for Stem Cell Research; the aim of which was for Dr. Gilbert to obtain training in the culture and differentiation of human and mouse ES cells during his sabbatical.
"Specification of Mammalian Replication Origins" Principle Investigator: David M. Gilbert, PhD Agency: NIH Type: RO1 (2 R01GM57233-05) Period: 9/01/02 - 8/31/07 Total Costs: $1,060,000.00 The goal of this grant is to test the hypothesis that the Origin Decision Point (ODP) is a point of global origin specification in mammalian cells, during which specific pre-RCs are selected for initiation of replication.
“Mapping Replication Origins in Human ES cells” Principle Investigator: David M. Gilbert, PhD Agency: NIH Type: Administrative Supplement to RO1 (2 R01GM57233-05) Period: 09/01/03 – 08/31/07 Total Costs: $340,000 The goal of this supplement is to map replication origins in human ES cells.
“Nuclear Organization and Stem Cell Commitment” Principle Investigator: David M. Gilbert, PhD Agency: American Cell Therapy Research Foundation/Stem Cell Research Foundation (SCRF) Type: Research grant (S2005-053) Period: 04/01/05 – 03/31/07 Total Costs: $100,000 The goal of this grant is to test the hypothesis that heritable changes in chromosome domain structure are established through changes in sub-nuclear position and replication timing that take place at key stages of cell commitment.
Completed Extra-Mural Post-Doctoral Support "Development of Promoter Shut-Off for the Study of Essential Mammalian Genes" Post-doctoral Scientist: Dr. Takayo Sasaki Agency: New York State Department of Health Breast Cancer Initiative Type: Post-doctoral award Period: 1/01/02 – 12/31/03 This project studied ways to develop genetic tools in cultured mammalian cells to study the origin recognition complex subunits.
Post-doctoral Scientist: Dr. Ichiro Hiratani Agency: Japan Society for the Promotion of Science Type: Post-doctoral award Period: 4/01/04 - 3/31/06 "Differentiation-coupled replication timing switches in embryonic stem cells" Post-Doctoral Scientist: Dr. Tomoki Yokochi Agency: Leukemia and Lymphoma Society Type: Special Fellow Period: 7/1/05-6/30/08 “The Effect of MeCP2 Deficiency on Epigenetic Regulation in Neuronal Lineages” Post-Doctoral Scientist: Dr. Ichiro Hiratani Agency: Rett Syndrome Research Foundation (RSRF) Period: 10/1/2006 – 9/30/2008 ACTIVE "Genome Plasticity During ES Cell Differentiation to Neural Lineages” Principle Investigator: David M. Gilbert, PhD Agency: NIH Type: RO1 (1R01GM083337-01A1) Period: 9/30/07 - 8/31/11 Total Costs: $1,100,000 The major goals of this project are to investigate epigenetic change during mouse ES neural differentiation. In particular, to examine chromatin changes at the Ptn/Chrm2 domain during the commitment to neural lineages and the role of the G9a histone methyltransferase in forming sub-nuclear compartments.
Administrative Supplement for Induced Pluripotent Stem Cell Biology Principle Investigator: David M. Gilbert, PhD Agency: NIH Type: 3R01GM083337-03S1 Period: 9/30/09 - 8/31/10 Total Costs: $110,250 This supplement tests the role of DNA replication timing as a barrier to iPS cell reprogramming.
“Replication Domain Organization During hESC Differentiation” Type: PPG (1P01GM085354-015319) Principle Investigator: David M. Gilbert, PhD / Stephen Dalton Agency: NIH Period: 8/1/08 – 7/31/13 Total costs for Gilbert Lab Portion: $1,656,575 This project examines changes in replication timing that occur during human ES cell differentiation to multiple lineages, how those changes relate to 3D changes in chromosome organization in the nucleus, and the organization of replication origins and forks across regions of chromosomes that experience replication timing changes.
Administrative Supplement for Human Embryonic Stem Cell Biology Principle Investigator: David M. Gilbert, PhD Agency: NIH Type: RFA-GM-08-003 (Parent grant 1PO1GM085354) Period: 9/30/09 - 8/31/11 Total Costs for Gilbert lab portion: $ 331,314 This supplement explores the structure of the human embryonic stem cell cycle.
"Regulation of chromosomal domain organization during ES cell differentiation" Post-Doctoral Scientist: Dr. Shin-Ichiro Takebayashi Agency: The Uehara Memorial Foundation Research Fellowship Period: 10/1/2009 to 9/30/2010